Isolation of detergent resistant microdomains from cultured neurons: detergent dependent alterations in protein composition
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چکیده
منابع مشابه
Convenient and rapid removal of detergent from glycolipids in detergent-resistant membrane microdomains.
Although detergents are often essential in protocols, they are usually incompatible with further biochemical analysis. There are several methods for detergent removal, but the procedures are complicated or suffer from sample loss. Here, we describe a convenient and rapid method for detergent removal from sialic acid-containing glycosphingolipids (gangliosides) and neutral glycolipids in deterge...
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Microdomains in the plasma membrane (PM) have been proposed to be involved in many important cellular events in plant cells. To understand the role of PM microdomains in plant cold acclimation, we isolated the microdomains as detergent-resistant plasma membrane fractions (DRMs) from Arabidopsis seedlings and compared lipid and protein compositions before and after cold acclimation. The DRM was ...
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Beckman SW41 rotor. After centrifugation, gradient fractions (1 ml) were collected from the top for further analysis. As shown in Figure 1a, the DRM fraction appeared as an opalescent band in the low density fraction of the gradient (fraction No. 6). The total protein in each fraction also can be used as an indicator of appropriate fractionation. Figure 1b shows that the majority of proteins in...
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The formation of lipid microdomains ("rafts") is presumed to play an important role in various cellular functions, but their nature remains controversial. Here we report on microdomain formation in isolated, detergent-resistant membranes from MDA-MB-231 human breast cancer cells, studied by atomic force microscopy (AFM). Whereas microdomains were readily observed at room temperature, they shrun...
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The pathways responsible for cell wall polysaccharide biosynthesis are vital in eukaryotic microorganisms. The corresponding synthases are potential targets of inhibitors such as fungicides. Despite their fundamental and economical importance, most polysaccharide synthases are not well characterized, and their molecular mechanisms are poorly understood. With the example of Saprolegnia monoica a...
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ژورنال
عنوان ژورنال: BMC Neuroscience
سال: 2010
ISSN: 1471-2202
DOI: 10.1186/1471-2202-11-120